duo set elisa development kits  (R&D Systems)

Journal: The Journal of Obstetrics and Gynaecology Research

Article Title: Impact of tumor necrosis factor‐alpha and lysophosphatidic acid on the behavior of ovarian cancer cells in a three‐dimensional collagen hydrogel

doi: 10.1111/jog.70026

Figure Lengend Snippet: VEGF secretion in response to TNF‐α or LPA at two different concentrations. The concentration of VEGF was measured with ELISA in SKOV‐3 (a and b), OVCAR‐8 (c and d), OVCAR‐5 (e and f), and OVCAR‐4 (g and h) cell lines grown in collagen gels for 2–8 days with the appropriate concentration of vehicle (control gels), TNF‐α or LPA. Protein concentrations of VEGF accumulated over 48 h in the culture medium are shown as mean + SEM from ≥3 independent experiments. Statistical significance p * <0.05; ** <0.01; **** <0.0001. LPA, lysophosphatidic acid; TNF‐α, tumor necrosis factor‐alpha; VEGF, vascular endothelial growth factor.

Article Snippet: Protein levels were measured using the human VEGF, human IL‐8, or human IL‐6 using the appropriate Duo SET ELISA development kits (R&D Systems, Minneapolis, MN, USA), following the manufacturer's protocols.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: The Journal of Obstetrics and Gynaecology Research

Article Title: Impact of tumor necrosis factor‐alpha and lysophosphatidic acid on the behavior of ovarian cancer cells in a three‐dimensional collagen hydrogel

doi: 10.1111/jog.70026

Figure Lengend Snippet: IL‐8 secretion in response to TNF‐α or LPA at two different concentrations. The concentration of IL‐8 was measured with ELISA in SKOV‐3 (a and b), OVCAR‐8 (c and d), OVCAR‐5 (e and f), and OVCAR‐4 (g and h) cell lines grown in collagen gels for 2–8 days with the appropriate concentration of vehicle (control gels), TNF‐α or LPA. Protein concentrations of IL‐8 accumulated over 48 h in the culture medium are shown as mean + SEM from ≥3 independent experiments. Statistical significance p * <0.05; ** <0.01; *** <0.001; **** <0.0001. IL‐8, interleukin‐8; LPA, lysophosphatidic acid; TNF‐α, tumor necrosis factor‐alpha.

Article Snippet: Protein levels were measured using the human VEGF, human IL‐8, or human IL‐6 using the appropriate Duo SET ELISA development kits (R&D Systems, Minneapolis, MN, USA), following the manufacturer's protocols.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: The Journal of Obstetrics and Gynaecology Research

Article Title: Impact of tumor necrosis factor‐alpha and lysophosphatidic acid on the behavior of ovarian cancer cells in a three‐dimensional collagen hydrogel

doi: 10.1111/jog.70026

Figure Lengend Snippet: IL‐6 secretion from cells that were stimulated by two concentrations of TNF‐α or LPA. The concentration of IL‐6 was measured with ELISA in SKOV‐3 (a and b), OVCAR‐8 (c and d), OVCAR‐5 (e and f), and OVCAR‐4 (g and h) cell lines grown in collagen gels for 2–8 days with the appropriate concentration of vehicle (control gels), TNF‐α or LPA. Protein concentrations of IL‐6 accumulated over 48 h in the culture medium are shown as mean + SEM from ≥3 independent experiments. Note the different scales in the left versus right columns. Statistical significance * <0.05 ** <0.01 *** <0.001 **** <0.0001. Results for OVCAR‐5 with LPA (f) were below detection. IL‐6, interleukin‐6; LPA, lysophosphatidic acid; TNF‐α, tumor necrosis factor‐alpha.

Article Snippet: Protein levels were measured using the human VEGF, human IL‐8, or human IL‐6 using the appropriate Duo SET ELISA development kits (R&D Systems, Minneapolis, MN, USA), following the manufacturer's protocols.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: BMC cancer

Article Title: Radiotherapy is enhanced by CPH:SA IL-1α microparticles in a murine HNSCC tumor model.

doi: 10.1186/s12885-025-13995-3

Figure Lengend Snippet: Fig. 1 Radiation induces cytokine release. A-C FaDu, Cal-27 and SQ20B HNSCC cells were exposed to 0 (Sham), 2, 4, and 8 Gy of X-ray radiation and analyzed for clonogenic survival (A), IL-1α (B) and IL-6 (C) 24 h after RT in cell culture media by ELISA. D-G: Cal-27 HNSCC cells were exposed to 0 (Sham), 2, 4, and 8 Gy of X-ray radiation then cell culture media harvested after the indicated timepoints for analysis of IL-1α (D), IL-1β (E) IL-1RA (F) and IL-6 (G) by ELISA. H: Cal-27 cells were pretreated with an IL-1 receptor antagonist (IL-1RA) and neutralizing antibodies against IL-1α (nIL-1α) and IL-1β (nIL-1β), exposed to 0 (Sham), 2, 4, and 8 Gy, then IL-6 measured in cell culture media by ELISA. Average values were normalized to Sham control and plotted as fold change. Bars represent the mean of n = 3 independent experiments. Error bars represent standard error from the mean. *p < .05; **p < .01; ***p < .001; ****p < .0001. ND: non-detectable

Article Snippet: Cell culture media was harvested at 24, 48 and 72 h following radiation for analysis of levels of IL-1α, IL-1β, IL-6 or IL-1RA using Human Duo Set ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer’s protocols and.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Control